Intraoperative Peritoneal Interleukin-6 Concentration Changes in Relation to the High-Mobility Group Protein B1 and Heat Shock Protein 70 Levels in Children Undergoing Cholecystectomy
Intraoperative Peritoneal Interleukin-6 Concentration Changes in Relation to the High-Mobility Group Protein B1 and Heat Shock Protein 70 Levels in Children Undergoing Cholecystectomy
Blog Article
The aim was the evaluation of IL-6 concentration in peritoneal lavage fluid of children which underwent cholecystectomy to ascertain if there is a difference in early inflammatory response depending on the type of surgical approach (open vs.laparoscopy).The analysis of high-mobility group protein B1 (HMGB1) and heat shock protein 70 (HSP70) was performed to find out if the source tablet buzz lightyear of IL-6 was related to tissue damage.
IL-6 concentration in peritoneal lavage fluid samples, obtained at the beginning and at the end of the laparoscopic (N=23) and open cholecystectomy (N=14), was tested with a routinely used electrochemiluminescence assay.The concentrations of HMGB1 and HSP70 were analyzed with the use of an ELISA method.Statistical analysis was performed using the STATISTICA PL release 12.
5 Program.The differences were assessed using the Mann-Whitney U test and Wilcoxon matched pairs test.Correlations were studied by using the Spearman correlation test.
Our results demonstrated significant peritoneal lavage fluid IL-6 concentration growth measured at the end of the cholecystectomy as compared to the beginning, regardless of the type of the procedure.IL-6 growth during open cholecystectomy was greater compared to laparoscopic cholecystectomy (62.51-fold vs.
3.19-fold).IL-6 concentration did not correlate with HMGB1 and HSP70, which indicate that the significant growth of this cytokine was not related to mechanical tissue damage due to surgical procedure.
A clinical significance of the study could be related to the fact cleveland browns scarf that the evaluation of IL-6 concentration in peritoneal lavage fluid may be useful to assess an early local inflammatory response.